Effect Of Binahong Leaf Extract ((Anredera Cordifolia (Tenore) Steenis) On Malondialdehyd, Hydroperoxide, Lipid, And Total Protein Levels In Cataract

Abstract

Objective:. A cataract is a condition where the lens loses its transparency or becomes cloudy; cataracts can occur due to oxidative stress due to the presence of free radicals. The free radical attack resulted in increased lipid hydroperoxide, MDA, and total insoluble protein. In this study, to overcome oxidative stress in diabetic cataract lenses, ethanol extract of binahong leaves was used, which has been reported to contain flavonoid compounds that can be used as antioxidants

Methods: This research was conducted at the Research Laboratory of the Faculty of Mathematics and Natural Sciences Unsyiah, The test materials used in this study were leaves of binahong Anredera cordifoia (Ten) steenis, liquid medium Nutrient broth (NB) distilled water, tween 80, 70% ethanol, HCl, Methanol, Mg, FeCl3, NaCl, H2SO4, EDTA, NaCl, KCl, MgCl2, NaHCO3, NaH(PO4)2, CaCl2, Glucose, Tris Buffer, Penicillin, Streptomycin, phenol, bovine serum albumin, butylated hydroxytoluene, sulfuric acid.Fresh goat eyeballs are collected from the slaughterhouse. Lenses were removed by extracapsular extraction and incubated in artificial aqueous humor (NaCl 140 mM, KCl 5 mM, MgCl2 2 mM, NaHCO3 0.5 mM, NaH (PO4) 2 0.5 mM, CaCl2 0.4 mM and Glucose 5.5 mM) at room temperature and pH 7.8 for 72 hours.   Principal Component Analysis (PCA) is a classical statistical technique that can be used to analyze a data set with many variables; in this study, the PCA technique was used to analyze MDA, LH, and total protein levels in regular lenses, diabetic lenses (cataracts), lenses after being treated with binahong leaf extract and lenses after being treated with quercetin (standard). PCA analysis in this study was carried out using the PYTHON application, using the Scikit-Learn Library, and visualized with the matplotlib and seaborn libraries.

Results: Based on the measurement results for each parameter, on the lipid hydroperoxide parameter, the lipid hydroperoxide level in normal lenses was 1.6611±0.01 g/mL, in diabetic cataract lenses 1.8508±0.01 g/mL, in diabetic cataract lenses after being given binahin extract at a dose of 100 mg. /mL lipid hydroperoxide levels became 1.5881±0.009 g/mL, while in binahong extract 200 mg/mL lipid hydroperoxide levels became T2 1.4390±0.01 g/mL. Measuring MDA levels in normal lenses showed results of 0.0252 ± 0.0015 g/mL, in diabetic cataract lenses 0.0562 ± 0.033 g/mL, after being given Binahing extract at a dose of 100 mg/mL the MDA levels became 0.0340 ± 0.0011 g/mL and at a dose of 200 mg/mL of MDA to 0.0300±0.0010 g/mL The same decreasing trend also occurred in total protein levels wherein normal lenses; the total protein level was 103.82±1.51 g/mL, in diabetic lenses it increased to 130.84±0.84 g/mL, total protein levels in diabetic cataract eyes were given 100 mg binahong extract. /mL dropped to 86.27±0.44 g/mL. and at binahong 200 mg/mL decreased to 73.92±0.62 g/mL

Conclusion: Binahong leaf extract gives good enough results to overcome the effects of free-racing attacks. The results of the PCA analysis showed that the levels of lipid hydroperoxide, MDA, and total protein tended to be different in both normal lenses, diabetic cataract lenses, and cataract lenses treated with binahong extract also with Quercetin.