Phytochemical Screening, Antioxidant, Antibacterial Activities And GC-MS Analysis Of Methanolic Extract Of Opuntia ficus-indica (L.) Mill. Fruit

Abstract

Background: Opuntia ficus-indica (L.) Mill., often known as Indian fig opuntia a drought-tolerant Cactaceae species that's grown in semi-arid and desert environments all over the world.
Objectives: The purpose of the research is to investigate the antioxidant, anti-inflammatory, antidiabetic, and antibacterial effects of O. ficus-indica fruit extracted in methanol.
Methods: The antioxidant activity of methanolic fruit extract of O. ficus-indica was assessed by DPPH (1,1-diphenyl-2-picrylhydrazyl), phosphomolybdenum assay, superoxide radical scavenging test, and ferric reduction power assay using ascorbic acid as the reference.
Results: Antibacterial activity was observed in gram-positive pathogens such as Staphylococcus aureus and Enterococcus faecalis, as well as gram-negative pathogens such as Escherichia coli and Pseudomonas aeruginosa. A membrane stabilization experiment was carried out utilizing blood saline to examine anti-inflammatory effects. Amylase inhibition, which employs iodine as a marker, is used to assess anti-diabetic activity.
Interpretation: Based on the GC-MS analysis, the presence of 4H-1-Benzopyran-4-one, 7-hydroxy-2-phenyl compound can be the reason for and this study demonstrated that future therapeutic development for a range of illnesses has promise.