Application of Zwitterionic Ion ChromatographyHydrophilic Interaction Liquid Chromatography for the Quantification and separation of Naringenin in Royal Jelly and Pollen extracts

Abstract

Natural products provide a large source of biological activities with a wide range of possible applications. In developed nations, natural
drugs not only fulfill primary health criteria, they also raise health-care costs as well. For this, they resort to naturally occurring compounds such as flavonoids. Flavonoids are nutrients from plants that can be non-toxic as well as beneficial to the human body when eaten in the form of fruits vegetables, beverage like tea and wine. Numerous health advantages have been related to the intake of food containing flavonoids. One of the most important flavonoids is naringenin. Naringenin is a compound of the flavonoids family found in all citrus fruit like oranges, lemons, grapes and bee products. Naringenin was separated in the ZIC®-HILIC column by acetonitrile (ACN) and sodium acetate (NaOAc) buffer as mobile phase constituents. The findings revealed that the HILIC mode was clear and efficient and could be used to identify royal jelly and pollen samples in naringenin. The calibration curve was developed for a wonderful type of commercial ZIC®- HILIC column and linear range (0.05-5 μgmL-1), RSD% (0.22 ± 0.045), LOD (0.008 μgmL-1), LOQ (0.024 μgmL-1).