Investigation of mast cell stabilization and antiulcer activity of protein extract of Perna viridis

Abstract

Objective: To study the mast cell stabilization and antiucler activity of protein extract
of Perna viridis using rodent models. Materials and Methods: The total protein from
P. viridis was extracted, purified, and screened for mast cell stabilization and antiulcer
activity. Intestinal mesentery of male rats was used to study the peritoneal mast cell
stabilization of protein extract of P. viridis. The rat intestinal mesentery was exposed to
10, 30, 100, 300, and 600 μg/mL of protein extract of P. viridis and the peritoneal mast
cell stabilization was compared with that of standard (ketotifen) at a dose of 20 μg/mL.
Antiulcer activity of protein extract of P. viridis was studied at the dose levels of 100,
200, and 400 mg/kg and the effect was compared with the omeprazole (20 mg/kg)
using ethanol-induced ulcer model. At the end of the study, ulcer index and percentage
of ulcer inhibition was calculated. Results: The total protein content in extract was
found to be 32 μg/mL. The protein extracts of P. viridis showed significant mast cell
stabilization only at high dose (600 μg/mL) and did not show any significant antiulcer
activity for doses of 100 and 200 mg/kg in administered animals, but the significant
antiulcer activity was observed at a dose of 400 mg/kg. Conclusion: The present study
findings suggests that the protein extract of P. viridis did not exhibited significant mast
cell stabilization and antiulcer activity at optimal doses.