Method Development And Validation For The Simultaneous Estimation Of Remdesivir In Bulk And Pharmaceutical Dosage Form And Stability Studies By Uplc

Abstract

An Easy, sensitive, specific and precise UPLC method for the pharmaceutical dose estimation of Remdesivir in tablet dosage form. Chromatogram was run through BEH (2.1 x 50mm, 1.7µm) Mobile phase containing 0.01N Kh2po4: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 0.3ml/min. Buffer used in this method was 0.01N Potassium dihydrogen phosphate buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 252.0nm. Retention time of Remdesivir were found to be 1.565 min. %RSD of the Remdesivir were and found to be 0.3. %RSD of Repeatably precision of Remdesivir were found to be 0.3. %Recovery was obtained as 99.87% for Remdesivir. %Assay was obtained as 99.58% for Remdesivir. LOD, LOQ values obtained from regression equation of Remdesivir were 0.33, 1.00. Regression equation of Remdesivir is y = 22602x + 1936. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.