Hepatoprotective, Antioxidant and Anti-inflammatory Actions of a Novel 5-Thiocyanauracil Compound and Ascorbic Acid against Paracetamol-Induced Hepatotoxicity in Female Rats

Abstract

The 5-Thiocyanauracil (TCU) is a novel synthetic molecule that has been proposed for its hepatoprotective, antioxidant, and antiinflammatory action. Overdosing on paracetamol has been linked to hepatic damage, which is a major health danger, especially for women. Our research's objective was to determine the induced hepatic, antioxidant, anti-inflammatory, and coagulopathy effects of 5-Thiocyanauracil against liver damage caused by paracetamol in rats. Using fifty female rats (10 each group), the following treatments were administered to the groups: Control (D.W or C) group, Paracetamol or PA (500 mg/kg/day) group, TCU (50 mg/kg/day), PA & Ascorbic Acid (AA) (AA 50 mg/kg/day), and PA+TCU group. For 25 days, treatments were given orally. Rats' hearts were directly used to draw blood samples. Prothrombin Time (PT) was evaluated using an auto-analyzer (Huma Clot Jonior), whereas protein carboxylase (PC), Glutathione peroxidase (GPX), and interleukin 6 (IL-6) were detected in serum and liver homogenate using the ELISA method.
TCU treatment dramatically increased GPX activity, decreased IL6 and PC levels as compared to rats given PA. This finding supports its
ability to reduce the PT/INR trend to undergo coagulopathy and to inhibit oxidation, inflammatory progressions caused by PA over dosage. In conclusion, TCU influenced potential hepatoprotective action that could have partially attributed to its anti-inflammatory and antioxidant properties in addition to PT/INR tests against PA-induced acute liver injury in a rat model.