The roles of vitamin E against free radicals during albendazole treatment in broiler chicken

Abstract

The present study was conducted to determine the effect of albendazole alone or in combination with vitamin E on antioxidant activity and histopathology, changes in the liver and kidney. Following oral administration of albendazole, 0.25 mg/kg body weight. Vitamin E 0.01 mg/kg body weight was used for 21 successive days on broiler chicken. The experiment was done on fifteen broiler chickens divided into three groups: one non-treated, two treated with albendazole o.25 mg/kg body weight, and three treated with albendazole in combination with vitamin E 0.01 mg/kg body weight. The blood sample and tissue were taken at the end of the experiment, 12hrs after the last dose. The experimental result revealed that the significant decrease of liver enzymes caused by albendazole like serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ATP), when compared with control group. the experimental revealed the decrease in kidney parameters like urea creatinine level caused by albendazole and finally there was a significant increase in antioxidant enzymes activity like CAT, SOD, GPX and a significant decrease in MDA. Histopathology results in liver-treated animals with albendazole in combination with vitamin E showed dilated, congested portal blood vessels (PBV, arrow), mild to moderate biliary proliferation (BP, arrow), portal round cell aggregation (RCA, arrow), and focal hepatocellular degeneration (HCD, arrow). Histopathology result of kidney-treated animal with albendazole in combination with vitamin E showing a mild peritubular edema (PTE arrow), focal tubular degeneration (TD arrow), tubular regeneration (TR arrow) glomerular lobulation and atrophy (GL arrow), beside interstitial cell's aggregation (RCA arrow). H&EX200, 400. Therefore, vitamin E should be taken with albendazole to decrease its effect.