Effect of Chlorogenic Acid on Sperm Parameters, DNA Integrity and Malondialdehyde of Carbamazepine - Consuming Epileptic Mice

Abstract

Background: Recently, administration of antiepilepsy drugs (AED) has become very common. This study aimed to assess the impacts of
chlorogenic acid(CA) on parameters, features of chromatin and DNA integrity of spermatozoa and assess the Malondialdehyde (MDA) in carbamazepine (CBZ)-consuming epileptic mice.
Materials and Methods: thirty-six adult male NMRI mice were randomly selected into six groups. Group Ι (Control), received basal diet;
group ΙΙ, pentylenetetrazol (PTZ); group ΙΙΙ, PTZ+carbamazepine; group ΙV, CA; group V, PTZ+CA; group VI, PTZ+CBZ+CA. Finally,
removal of caudal epididymis was done and placed in 1mL Ham'sF10 medium at 37C for 15 min. Then, analysis of sperm parameters was executed. Toluidine blue (TB), chromomycinA3 (CMA3), aniline blue (AB) and TUNLE were applied for assessment of sperm chromatin quality. Also, lipid peroxidation and total antioxidant capability were measured by MDA test.
Results: A significant increase was evident in sperm parameters in the CA, PTZ+CA and PTZ+CBZ+CA groups in comparison with the
control and PTZ groups. About AB, TB, CMA3 and TUNEL tests, there was a significant increase in the PTZ group in comparison with the
PTZ group. MDA test showed significant decreasing in the PTZ group compared to all groups. Also, the PTZ+CBZ+CA group had a
significant increase compared to the control, PTZ, PTZ+CBZ and PTZ+CA groups
Conclusion: Although epilepsy can have deleterious effects on sperm fertility and DNA integrity, the CBZ and CA administration has
positive result on sperm parameters, sperm chromatin abnormalities, and MDA in mice with epilepsy.