SPERM PREPARATION - SWIM UP, DENSITY GRADIENT, MICROFLUIDIC SPERM SORTER METHOD & MAGNETICACTIVATED CELL SORTING METHOD – A COMPARATIVE ANALYSIS

Abstract

Infertility is a major concern for many couples who want children of their own. The cause of infertility may be with either of the partners. The use of Assisted Reproductive Techniques (ART) is the solution for those couples who have infertility issues. In this article, the main focus is on male infertility. In order to determine the acceptability of processed semen for breeding purposes, a precise evaluation of semen fertilizing capacity is critical. A solid sperm preparation process yields a sample with excellent viability and motility, as well as taking into account other factors like Capacitation and apoptosis, which can affect the capacity to fertilize an oocyte. In order to determine the acceptability of processed semen for breeding purposes, a precise evaluation of semen fertilizing capacity is critical. A solid sperm preparation process yields a sample with excellent viability and motility, as well as taking into account other factors like Capacitation and apoptosis, which can affect the capacity to fertilize an oocyte. This article provides a comparison of four different sperm processing/washing techniques of swim up, density gradient, microfluidic cell sorter method & magnetically activated cell sorting method. The various aspects of each of these techniques are discussed.