Quality Analysis Of Traditional Medicinal Plants With Potential In Management Of Liver Cancer

Abstract

Liver and its dysfunctions are well defined in Indigenous systems of medicine. Various herbal drugs are documented for effective
treatment of liver disorders. Some of these are highly effective and have highpotential for application in the treatment of hepatocellular
carcinoma. Standardization of herbal drugs is an essential requirement to persuade scientific studies. In this study, we have provided
quality parametersfor fifteen selected constituent herbs based on their physicochemical and thin layer chromatographic parameters and
their correlation with cytotoxic effect on HepG2 cell line.Quality standards for these 15 plant ingredients will contribute to further
develop a standardised product.
Methods Fifteen plants sampleswere procured from at least three different sources. These were authenticated and analysed for
organoleptic, physicochemical properties and TLC.The aqueous extracts of all plant drugs were evaluated for cytotoxic effect on HepG2
cell line using MTT assay.
Results The physicochemical parameters and Rf values were compared with the standards.The results were correlated with cytotoxic
potential of the drug on HepG2 cell line. The study determined quality profile of plant drug samples matching with pharmacopeial
standards and having maximum efficacy for cytotoxic potential on HepG2 cell line.
Conclusion The study is essentialto determine the quality and purity of the crude plant drug (raw material) as standards for selection of
plants and for development of a monograph for the product formulation,to be used in the management of hepatocellular carcinoma. It
is also of value in laying down of standards for a multi-ingredient formulation.