Screening And Characterization Of Biosurfactant-Producing Bacteria Isolated From Oil-Contaminated Soil

Abstract

Some bacterial isolates make biosurfactants, which are chemical substances that help oil biodegrade. 13 gram positive and gram negative bacteria isolates were isolated from various sources of oil polluted soil and identified using various ways before being submitted to select the active isolate for biosurfactant synthesis. Several approaches were used to screen isolates for biosurfactant-producing bacteria in this investigation. generating biosurfactants On hemolysis activity, cetyl trim ethyl ammonium bromide (CTAB) agar, oil spreading test, drop collapse assay, emulsification index, BATH (Bacterial adhesion to hydrocarbons) assay, and foaming activity. The biosurfactant productivity was shown to be highest in (Kocuria kristinae, Staphylococcus warneri, Staphylococcus haemolyticus, Micrococcus luteus, Delftia acidovorans, Pseudomonas putida, Pseudomonas aeruginosa, and Pseudomonas stutzeri).