The Occurrence Of Genetic Variation In B.Abortus Isolated From Sheep In Babylon Province, Iraq

Abstract

The samples collected from sheep's blood from(Al-Mihnawiya Village) in Babylon province , middle of Hilla , Iraq in the end of May month-2021. This investigation aim to knowledge presence types of Brucella spp. in the sheep's blood. Brucella is pathogen of  zoonotic  disease between animals and human. The bacteria isolated and identification by pcr. The results revealed presence the bacteria in all the blood samples and after senting these samples to DNA sequence noticed  two species of bacteria included B.melitensis and B.abortus where compared these local samples with world researchs in NCBI by phylogenetic tree.DNA sequencing  for B.melitensis and B.abortus include substituation mutation ( transversion and transition)  and at compared these results for genotype with phenotype that include (WBC, granulocyte and lymphocyte) by statistical analysis don’t have significant differences (P>0.05%) in B.melitensis but there is significant differences(P<0.05%)  in B.abortus ,  this mean existing genetic variety  in this bacteria.