Comparison the effects of Cds-Au nanoparticles on viability of normal and cancer cell lines and expression of P53 genes

Abstract

Background and Objectives: Nanoparticles have been given a solution to many medical and industrial problems and other applications
through their small size and ease of controlling their physical and chemical properties, as well as merging them with other materials to give them different properties used in multiple fields, including the field of medicine. This study aims to test the toxicity of Cds-Au NPS on cancerous and normal cell lines and apoptosis gene expression.
Results: IC50 value of Cds-Au NPS in Vero (normal cell model) 19.50 µg /ml while in MCF-7 (cancer cell model) 57 µg /ml. Effect of
embedded nanoparticles on the phenotypical morphology of cell lines by AO and E & H The results show the degradation of the cytoplasm in cells and apoptotic cells vacuole degeneration, cell-free spaces, the remains of the dissolved cells, the floating cells, and enlarged and thickened nucleus. When the cell lines were treated with Cds-Au NPS, it showed an effect of elevation in p53 expression in treated cancer cells more than 14 times that of treated normal cells. While PTEN gene and P21 gene is higher in the cancer cells mor than normal cells.
Conclusions: CdS-Au NPs Show high toxicity effect on both cancer and normal cell lines also activate the P21, P53 and PTEN gene in
MCF-7 cells