ANTI PROLIFERATIVE EFFECT OF ENDOGENOUS DOPAMINE REPLICA IN HUMAN LUNG CANCER CELLS (A549) VIA PI3K AND AKT SIGNALLING MOLECULES

Abstract

Background: Lung cancer is one of the most common primary malignant tumour types in numerous countries.
Non-small cell lung cancer (NSCLC) is one of the deadliest cancers which is present in this world. Dopamine,
which is secreted in the brain, is a neurotransmitter as it acts as a chemical messenger between neurons. Dopamine
receptor D2(DRD2) has multiple roles in clinical progression of NSCLC and is also useful in functional
maintenance of cancer cells. It has been proved that regular exercise improves quality of life in cancer patients.The
main aim of this study is to assess the anti proliferative effect of endogenous dopamine replicas in human lung
cancer cells(A549) through PI3K and AKT signalling pathway..
Materials and Methods: Human lung cancer cell line (A549) was brought from the National Centre for Cell
Sciences (NCCS),Pune,India. Cell viability test was done by MTT assay. Gene expression analysis was done by
Real Time-PCR. The obtained data were analysed statistically by one-way analysis of variance and Duncan’s
multiple range test with Graph Pad Prism version 5 to analyse the significance. The significance was considered
at p<0.05 level in Duncan’s test.
Results: The Bcl-2, PI3K and Akt gene expression is reduced on induction of 100 and 200 µg/ml (dosage) of
Dopamine with significant difference in comparison with control. Exercise induced Dopamine caused a marked
increase in cell death in a dose dependent manner. At the end of 48 hours, maximum inhibition was at 100 and
200 μg/ml. Dopamine reduces the expression of Bcl-2, PI3K and Akt compared to the control cells.
Conclusion: From the obtained results and within the limits of the study, it can be concluded that exercise induced
endogenous Dopamine has a massive anti-cancer potential against Lung cancer by interrupting PI3K/Akt
signalling molecules as an anti-proliferative agent. Future studies should be conducted on a large scale to make
this context evident.