Spectrophotometric Determination Of Anti-Ulcer Drug (Cimetidine) By 2.4-Dinitrophenylhydrazine Reagent

Abstract

An innovative, simple, and sensitive spectrophotometric method was described for the determination of cimetidine in both its pure
form and in pharmaceutical formulations. By oxidizing 2.4-dinitrophenylhydrazine (2.4-DNPH) and mixing it with cimetidine in an
alkaline solution, a brightly colored chromogen with a maximum absorption wavelength of 586 nm is created. After that,
spectrophotometry was used to quantify the amount of cimetidine. Other analytical criteria, such as the ideal reaction circumstances,
were evaluated. These criteria included variables like the volumes of 2.4-DNPH and potassium iodate, the amount of base, the
coupling reaction time, temperature, and the order in which the constituents of the final product are added. Beer's law is applied to
concentrations of (25–250) µg/mL-1 with correlation coefficient (R2
) = 0.9964, molar absorptivity 353.276 (L.mol-1
.cm-1
), and
Sandell's law. The recommended method for locating cimetidine in pharmaceutical formulations worked well.