The Effect of Butenyl Isothiocyanate on Angiogenesis Ex Vivo and in Vivo Animal Study

Abstract

Background: Angiogenesis is the development of new capillary blood vessels from existing ones that are required for several physiological and pathological processes. Butenyl isothiocyanate is a member of isothiocyanate which has drawn more attention Since they have a lot of biological activity and are given credit for the benefits of cruciferous vegetable consumption on cancer. Additionally, acetylsalicylic acid the non-steroidal anti-inflammatory medicine significantly lowers the angiogenesis of cancer. Objective: The study aimed to identify the antiangiogenic activity of butenyl isothiocyanate alone and in combination with acetylsalicylic acid and to investigate the synergistic and/or additive effect of butenyl isothiocyanate and acetylsalicylic acid as anti-angiogenic compounds. Methods: 12-14 weeks-old Albino male rats were used for the study. The tested substances butenyl isothiocyanate and acetylsalicylic acid were serially diluted. An ex vivo rat aorta ring experiment has been used to examine Butenyl isothiocyanate's potential antiangiogenic properties. Using an in vivo chorioallantoic membrane (CAM) experiment, the butenyl isothiocyanate-induced zone of blood vessel inhibition was measured and the collected data were statistically analyzed.

Results: The result of this study showed that there is a significant antiangiogenic activity of butenyl isothiocyanate and acetylsalicylic
acid and there is an additive antiangiogenic effect of butenyl isothiocyanate and acetylsalicylic acid combination in ex vivo rat aorta ring antiangiogenesis assay (P<0.05). Each butenyl isothiocyanate and acetylsalicylic acid showed significant anti-angiogenesis activity in the in vivo CAM assay.

Conclusion: The present study revealed that each butenyl isothiocyanate and acetylsalicylic acid exhibited significant antiangiogenesis activity in both ex vivo and in vivo models of angiogenesis.